p ip3r ser1756  (Cell Signaling Technology Inc)

Journal: Frontiers in Pharmacology

Article Title: Bmal1 haploinsufficiency impairs fear memory and modulates neuroinflammation via the 5-HT2C receptor

doi: 10.3389/fphar.2024.1422693

Figure Lengend Snippet: Detailed antibody information.

Article Snippet: p-IP3 , Cell Signaling Technology , #12243 , 1:1000.

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Bmal1 haploinsufficiency impairs fear memory and modulates neuroinflammation via the 5-HT2C receptor

doi: 10.3389/fphar.2024.1422693

Figure Lengend Snippet: Bmal1 +/− mice show abnormal neurotransmitter levels and molecular signals. (A–F) Levels of neurotransmitters in the PFC of Bmal1 +/+ and Bmal1 +/− mice after cued fear conditioning paradigm. 5-HT (A) , dopamine (B) , GABA (C) , glutamate (D) , norepinephrine (E) , and acetylcholine (F) . n = 7 mice. Data are reported as mean ± SEM. Unpaired t-test. ns, no significance; *p < 0.05; **p < 0.01. (G, H) Representative Western blot images (G) and protein levels of IDO, 5-HT2CR, SIRT1, TPH2, TH, DAT, p-IP3, p-PKC, and p-CREB in the PFC of Bmal1 +/+ and Bmal1 +/− mice after the cued fear conditioning paradigm (H) . n = 4–6 mice. Data are reported as mean ± SEM. Unpaired t-test. ns, no significance; *p < 0.05; **p < 0.01.

Article Snippet: p-IP3 , Cell Signaling Technology , #12243 , 1:1000.

Techniques: Western Blot

Journal: Aging

Article Title: Sevoflurane impedes neuropathic pain by maintaining endoplasmic reticulum stress and oxidative stress homeostasis through inhibiting the activation of the PLCγ/CaMKII/IP3R signaling pathway.

doi: 10.18632/aging.206001

Figure Lengend Snippet: Figure 5. Sevoflurane could relieve calcium influx and ER stress. (A) Protein bands of p-PLCγ, p-CaMKII, p-IP3R, GRP78 and GRP94 in Sham group, Model group and Sevoflurane group, (B) relative protein expressions of p-PLCγ, p-CaMKII, p-IP3R, GRP78 and GRP94 in Sham group, Model group and Sevoflurane group. Sham group vs. Model group, Model group vs. Sevoflurane group, Control group vs. Sevoflurane group, **P<0.01, N=8.

Article Snippet: Thereafter, the membrane was blocked for 2h at room temperature, and reacted with corresponding primary antibodies against phosphorylated (p)-PLCγ (Abcam, UK, ab81284, 1: 2000), phosphorylated calcium/ calmodulin-dependent protein kinase II (p-CaMKII) www.aging-us.com 9 AGING (Abcam, ab171095, 1: 2000), p-IP3R (Cell Signaling, USA, 8548, 1: 1000), glucose-regulated protein 78 (GRP78) (Abcam, ab171089, 1: 1000), GRP94 (Abcam, ab210960, 1: 1000), P22 (Abcam, ab75941, 1: 1000), P47 (Abcam, ab308256, 1: 1000), nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) (Abcam, ab263899, 1: 1000), IL-1β (Abcam, ab283818, 1: 1000), TNF-α (Abeam, ab205587, 1: 1000) and GAPDH (Abcam, ab2293, 1: 2500) at 4° C overnight.

Techniques: Control

Journal: Aging

Article Title: Sevoflurane impedes neuropathic pain by maintaining endoplasmic reticulum stress and oxidative stress homeostasis through inhibiting the activation of the PLCγ/CaMKII/IP3R signaling pathway.

doi: 10.18632/aging.206001

Figure Lengend Snippet: Figure 7. In vitro experiments verified that sevoflurane could mediate the PLCγ/CaMKII/IP3R signaling pathway to inhibit ER stress, oxidative stress and inflammatory response. (A) Protein bands of p-PLCγ, p-CaMKII, p-IP3R, GRP78, P22 and NLRP3 in each group, (B) relative protein expressions of p-PLCγ, p-CaMKII, p-IP3R, GRP78, P22 and NLRP3 in each group. Sham group vs. Model group, Model group vs. Sevoflurane group, Sevoflurane group vs. Sevoflurane + IP3R Inhibitor group, **P<0.01, N=3.

Article Snippet: Thereafter, the membrane was blocked for 2h at room temperature, and reacted with corresponding primary antibodies against phosphorylated (p)-PLCγ (Abcam, UK, ab81284, 1: 2000), phosphorylated calcium/ calmodulin-dependent protein kinase II (p-CaMKII) www.aging-us.com 9 AGING (Abcam, ab171095, 1: 2000), p-IP3R (Cell Signaling, USA, 8548, 1: 1000), glucose-regulated protein 78 (GRP78) (Abcam, ab171089, 1: 1000), GRP94 (Abcam, ab210960, 1: 1000), P22 (Abcam, ab75941, 1: 1000), P47 (Abcam, ab308256, 1: 1000), nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) (Abcam, ab263899, 1: 1000), IL-1β (Abcam, ab283818, 1: 1000), TNF-α (Abeam, ab205587, 1: 1000) and GAPDH (Abcam, ab2293, 1: 2500) at 4° C overnight.

Techniques: In Vitro

Journal: Aging

Article Title: Sevoflurane impedes neuropathic pain by maintaining endoplasmic reticulum stress and oxidative stress homeostasis through inhibiting the activation of the PLCγ/CaMKII/IP3R signaling pathway.

doi: 10.18632/aging.206001

Figure Lengend Snippet: Figure 8. Sevoflurane maintained ER stress and oxidative stress homeostasis by inhibiting the activation of the PLCγ/CaMKII/IP3R signaling pathway, thereby repressing neuropathic pain.

Article Snippet: Thereafter, the membrane was blocked for 2h at room temperature, and reacted with corresponding primary antibodies against phosphorylated (p)-PLCγ (Abcam, UK, ab81284, 1: 2000), phosphorylated calcium/ calmodulin-dependent protein kinase II (p-CaMKII) www.aging-us.com 9 AGING (Abcam, ab171095, 1: 2000), p-IP3R (Cell Signaling, USA, 8548, 1: 1000), glucose-regulated protein 78 (GRP78) (Abcam, ab171089, 1: 1000), GRP94 (Abcam, ab210960, 1: 1000), P22 (Abcam, ab75941, 1: 1000), P47 (Abcam, ab308256, 1: 1000), nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) (Abcam, ab263899, 1: 1000), IL-1β (Abcam, ab283818, 1: 1000), TNF-α (Abeam, ab205587, 1: 1000) and GAPDH (Abcam, ab2293, 1: 2500) at 4° C overnight.

Techniques: Activation Assay